RNA-Seq at different stages

Meiosis RNA-Seq data

RNA-Seq data were generated in meiosis process of Tetrahymena thermophila conjugation, including:
1) the WT (B2086 X CU428) and SPO11 knockout conjugation cells were collected at 2, 3, 4 and 5 hours;
2) the WT (B2086 X CU428) and CYC17 knockout conjugation cells were collected at 4, 5, 6 and 7 hours;
3) the WT (B2086 X CU428) and CYC2 knockout conjugation cells were collected at 2, 2.5, 3 and 3.5 hours;

RNA samples extracted as described in the "Sample Preparation" page. mRNA was purified and sequenced using Illumina Hiseq 2000 sequencer by 100 X2 (Paired-end mode) or Hiseq 4000 sequencer by 150 X2.

To search, type your Gene ID using the 2008 genome annotation.

Notes for seaching the RNA-Seq data at Meiosis

The Gbrowse automaticly show the scale bar for the RPKM vale of each gene. If you want to show different genes in the same scale, please follow the method in the bellow image. If the explorer of your computer can not automaticly display the RPKM value tracks, please empty the cookies of the explorer, or go to the "Select Tracks" page of Gbrowse to select all the RPKM tracks.